Real-time Polymerase Chain Reaction:Article Title: Cancer cell-derived sialylated IgG interacting with Siglec-7/9/10 is a potential immunotherapeutic target in pancreatic cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human IFN-γ Peprotech Cat# 300-02-20 Human M-CSF Peprotech Cat# 300-25-2 Mouse M-CSF Peprotech Cat# 315-03-5 Human EGF Peprotech Cat# AF-100-15 Human FGF-basic (FGF-2/bFGF) Peprotech Cat# 100-18B B27 Gibco Cat# 17504044 Collagenase IV Solarbio Cat# C8160 DNase I Solarbio Cat# D8070 PNGase F NEB Cat# P0704 α 2-3,6,8 Neuraminidase NEB Cat# P0720 α 2-3 Neuraminidase NEB Cat# P0743 Human Siglec-9, His Tag ACRO Biosystems Cat# SI9-H52H4 CFSE Thermo Fisher Scientific Cat# C34554 pHrodo Red succinimidyl ester Thermo Fisher Scientific Cat# P36600 Hoechst Solarbio Cat# C0031 TRIzol Reagent Life Technology Cat# 15596018 1×Hieff qPCR SYBR Green Master Mix Yeasen Biotechnology Cat# 11203ES08 PEI Reagent Polysciences Cat# 23966 Fetal Bovine Serum HyClone Cat# SH30396.02 RPMI 1640 Gibco Cat# 31800-022 DMEM Gibco Cat# 12100061 Cell lysates Leagene Cat# PS0013 10mM sodium acetate pH4.5 Cytiva Cat# BR-1003-50 10mM sodium acetate pH5.5 Cytiva Cat# BR-1003-52 10mM Glycine-HCl Cytiva Cat# BR-1003-56 50mM NaOH Cytiva Cat# BR-1003-58 PBS-P+ Buffer 10× Cytiva Cat# 28995084 Critical commercial assays Opal 6-Plex Manual Detection Kit Akoya Biosciences Cat# NEL811001KT DAB Detection Kit Gene Tech Cat# GK600705 Protein G Sepharose 4 Fast Flow column GE Healthcare Cat# 17-0618-01 CNBr activated Sepharose 4B71-7086-00 AF GE Healthcare Cat# 71-7086-00 AF EasySep Human CD14 Positive Selection Kit II StemCell Technologies Cat# 17858 RevertAid First Stand cDNA Synthesis Kit Thermo Fisher Scientific Cat# K1622 Dynabeads His-Tag Isolation and Pulldown Thermo Fisher Scientific Cat# 10103D Clophosome-A and Control Liposomes FormuMax Cat# F70101C-NC-10 Amino Conjugation Kit Cytiva Cat# BR-1000-50 EZ-Link Sulfo-NHS-LC-Biotinylation Kit Thermo Fisher Scientific Cat# 21435 Lipofectamine 3000 Thermo Fisher Scientific Cat# L-3000015 LEGEND MAX Mouse IL-10 ELISA Kit Biolegend Cat# 431417 Human/Mouse TGF beta-1 Uncoated ELISA Kit Thermo Fisher Scientific Cat# 88-8350-88 Mouse IL-6 Uncoated ELISA Kit Thermo Fisher Scientific Cat# 88-7064-88 Deposited data PDAC scRNA-Seq Peng et al.35 GSA: CRA001160 RNA seq of KPC treated with TGF-β1 Hou et al.39 GSE149130 Original proteomic data Tables S5 and S6 This paper Tables S5 and S6 (Continued on next page) e3 Cell Reports Medicine 7, 102660, March 17, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines 293FT Cell Resource Center, Peking Union Medical College RRID: CVCL_6911 hTERT-HPNE ATCC RRID: CVCL_C466
SYBR Green Assay:Article Title: Cancer cell-derived sialylated IgG interacting with Siglec-7/9/10 is a potential immunotherapeutic target in pancreatic cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human IFN-γ Peprotech Cat# 300-02-20 Human M-CSF Peprotech Cat# 300-25-2 Mouse M-CSF Peprotech Cat# 315-03-5 Human EGF Peprotech Cat# AF-100-15 Human FGF-basic (FGF-2/bFGF) Peprotech Cat# 100-18B B27 Gibco Cat# 17504044 Collagenase IV Solarbio Cat# C8160 DNase I Solarbio Cat# D8070 PNGase F NEB Cat# P0704 α 2-3,6,8 Neuraminidase NEB Cat# P0720 α 2-3 Neuraminidase NEB Cat# P0743 Human Siglec-9, His Tag ACRO Biosystems Cat# SI9-H52H4 CFSE Thermo Fisher Scientific Cat# C34554 pHrodo Red succinimidyl ester Thermo Fisher Scientific Cat# P36600 Hoechst Solarbio Cat# C0031 TRIzol Reagent Life Technology Cat# 15596018 1×Hieff qPCR SYBR Green Master Mix Yeasen Biotechnology Cat# 11203ES08 PEI Reagent Polysciences Cat# 23966 Fetal Bovine Serum HyClone Cat# SH30396.02 RPMI 1640 Gibco Cat# 31800-022 DMEM Gibco Cat# 12100061 Cell lysates Leagene Cat# PS0013 10mM sodium acetate pH4.5 Cytiva Cat# BR-1003-50 10mM sodium acetate pH5.5 Cytiva Cat# BR-1003-52 10mM Glycine-HCl Cytiva Cat# BR-1003-56 50mM NaOH Cytiva Cat# BR-1003-58 PBS-P+ Buffer 10× Cytiva Cat# 28995084 Critical commercial assays Opal 6-Plex Manual Detection Kit Akoya Biosciences Cat# NEL811001KT DAB Detection Kit Gene Tech Cat# GK600705 Protein G Sepharose 4 Fast Flow column GE Healthcare Cat# 17-0618-01 CNBr activated Sepharose 4B71-7086-00 AF GE Healthcare Cat# 71-7086-00 AF EasySep Human CD14 Positive Selection Kit II StemCell Technologies Cat# 17858 RevertAid First Stand cDNA Synthesis Kit Thermo Fisher Scientific Cat# K1622 Dynabeads His-Tag Isolation and Pulldown Thermo Fisher Scientific Cat# 10103D Clophosome-A and Control Liposomes FormuMax Cat# F70101C-NC-10 Amino Conjugation Kit Cytiva Cat# BR-1000-50 EZ-Link Sulfo-NHS-LC-Biotinylation Kit Thermo Fisher Scientific Cat# 21435 Lipofectamine 3000 Thermo Fisher Scientific Cat# L-3000015 LEGEND MAX Mouse IL-10 ELISA Kit Biolegend Cat# 431417 Human/Mouse TGF beta-1 Uncoated ELISA Kit Thermo Fisher Scientific Cat# 88-8350-88 Mouse IL-6 Uncoated ELISA Kit Thermo Fisher Scientific Cat# 88-7064-88 Deposited data PDAC scRNA-Seq Peng et al.35 GSA: CRA001160 RNA seq of KPC treated with TGF-β1 Hou et al.39 GSE149130 Original proteomic data Tables S5 and S6 This paper Tables S5 and S6 (Continued on next page) e3 Cell Reports Medicine 7, 102660, March 17, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines 293FT Cell Resource Center, Peking Union Medical College RRID: CVCL_6911 hTERT-HPNE ATCC RRID: CVCL_C466
Selection:Article Title: Cancer cell-derived sialylated IgG interacting with Siglec-7/9/10 is a potential immunotherapeutic target in pancreatic cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human IFN-γ Peprotech Cat# 300-02-20 Human M-CSF Peprotech Cat# 300-25-2 Mouse M-CSF Peprotech Cat# 315-03-5 Human EGF Peprotech Cat# AF-100-15 Human FGF-basic (FGF-2/bFGF) Peprotech Cat# 100-18B B27 Gibco Cat# 17504044 Collagenase IV Solarbio Cat# C8160 DNase I Solarbio Cat# D8070 PNGase F NEB Cat# P0704 α 2-3,6,8 Neuraminidase NEB Cat# P0720 α 2-3 Neuraminidase NEB Cat# P0743 Human Siglec-9, His Tag ACRO Biosystems Cat# SI9-H52H4 CFSE Thermo Fisher Scientific Cat# C34554 pHrodo Red succinimidyl ester Thermo Fisher Scientific Cat# P36600 Hoechst Solarbio Cat# C0031 TRIzol Reagent Life Technology Cat# 15596018 1×Hieff qPCR SYBR Green Master Mix Yeasen Biotechnology Cat# 11203ES08 PEI Reagent Polysciences Cat# 23966 Fetal Bovine Serum HyClone Cat# SH30396.02 RPMI 1640 Gibco Cat# 31800-022 DMEM Gibco Cat# 12100061 Cell lysates Leagene Cat# PS0013 10mM sodium acetate pH4.5 Cytiva Cat# BR-1003-50 10mM sodium acetate pH5.5 Cytiva Cat# BR-1003-52 10mM Glycine-HCl Cytiva Cat# BR-1003-56 50mM NaOH Cytiva Cat# BR-1003-58 PBS-P+ Buffer 10× Cytiva Cat# 28995084 Critical commercial assays Opal 6-Plex Manual Detection Kit Akoya Biosciences Cat# NEL811001KT DAB Detection Kit Gene Tech Cat# GK600705 Protein G Sepharose 4 Fast Flow column GE Healthcare Cat# 17-0618-01 CNBr activated Sepharose 4B71-7086-00 AF GE Healthcare Cat# 71-7086-00 AF EasySep Human CD14 Positive Selection Kit II StemCell Technologies Cat# 17858 RevertAid First Stand cDNA Synthesis Kit Thermo Fisher Scientific Cat# K1622 Dynabeads His-Tag Isolation and Pulldown Thermo Fisher Scientific Cat# 10103D Clophosome-A and Control Liposomes FormuMax Cat# F70101C-NC-10 Amino Conjugation Kit Cytiva Cat# BR-1000-50 EZ-Link Sulfo-NHS-LC-Biotinylation Kit Thermo Fisher Scientific Cat# 21435 Lipofectamine 3000 Thermo Fisher Scientific Cat# L-3000015 LEGEND MAX Mouse IL-10 ELISA Kit Biolegend Cat# 431417 Human/Mouse TGF beta-1 Uncoated ELISA Kit Thermo Fisher Scientific Cat# 88-8350-88 Mouse IL-6 Uncoated ELISA Kit Thermo Fisher Scientific Cat# 88-7064-88 Deposited data PDAC scRNA-Seq Peng et al.35 GSA: CRA001160 RNA seq of KPC treated with TGF-β1 Hou et al.39 GSE149130 Original proteomic data Tables S5 and S6 This paper Tables S5 and S6 (Continued on next page) e3 Cell Reports Medicine 7, 102660, March 17, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines 293FT Cell Resource Center, Peking Union Medical College RRID: CVCL_6911 hTERT-HPNE ATCC RRID: CVCL_C466
cDNA Synthesis:Article Title: Cancer cell-derived sialylated IgG interacting with Siglec-7/9/10 is a potential immunotherapeutic target in pancreatic cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human IFN-γ Peprotech Cat# 300-02-20 Human M-CSF Peprotech Cat# 300-25-2 Mouse M-CSF Peprotech Cat# 315-03-5 Human EGF Peprotech Cat# AF-100-15 Human FGF-basic (FGF-2/bFGF) Peprotech Cat# 100-18B B27 Gibco Cat# 17504044 Collagenase IV Solarbio Cat# C8160 DNase I Solarbio Cat# D8070 PNGase F NEB Cat# P0704 α 2-3,6,8 Neuraminidase NEB Cat# P0720 α 2-3 Neuraminidase NEB Cat# P0743 Human Siglec-9, His Tag ACRO Biosystems Cat# SI9-H52H4 CFSE Thermo Fisher Scientific Cat# C34554 pHrodo Red succinimidyl ester Thermo Fisher Scientific Cat# P36600 Hoechst Solarbio Cat# C0031 TRIzol Reagent Life Technology Cat# 15596018 1×Hieff qPCR SYBR Green Master Mix Yeasen Biotechnology Cat# 11203ES08 PEI Reagent Polysciences Cat# 23966 Fetal Bovine Serum HyClone Cat# SH30396.02 RPMI 1640 Gibco Cat# 31800-022 DMEM Gibco Cat# 12100061 Cell lysates Leagene Cat# PS0013 10mM sodium acetate pH4.5 Cytiva Cat# BR-1003-50 10mM sodium acetate pH5.5 Cytiva Cat# BR-1003-52 10mM Glycine-HCl Cytiva Cat# BR-1003-56 50mM NaOH Cytiva Cat# BR-1003-58 PBS-P+ Buffer 10× Cytiva Cat# 28995084 Critical commercial assays Opal 6-Plex Manual Detection Kit Akoya Biosciences Cat# NEL811001KT DAB Detection Kit Gene Tech Cat# GK600705 Protein G Sepharose 4 Fast Flow column GE Healthcare Cat# 17-0618-01 CNBr activated Sepharose 4B71-7086-00 AF GE Healthcare Cat# 71-7086-00 AF EasySep Human CD14 Positive Selection Kit II StemCell Technologies Cat# 17858 RevertAid First Stand cDNA Synthesis Kit Thermo Fisher Scientific Cat# K1622 Dynabeads His-Tag Isolation and Pulldown Thermo Fisher Scientific Cat# 10103D Clophosome-A and Control Liposomes FormuMax Cat# F70101C-NC-10 Amino Conjugation Kit Cytiva Cat# BR-1000-50 EZ-Link Sulfo-NHS-LC-Biotinylation Kit Thermo Fisher Scientific Cat# 21435 Lipofectamine 3000 Thermo Fisher Scientific Cat# L-3000015 LEGEND MAX Mouse IL-10 ELISA Kit Biolegend Cat# 431417 Human/Mouse TGF beta-1 Uncoated ELISA Kit Thermo Fisher Scientific Cat# 88-8350-88 Mouse IL-6 Uncoated ELISA Kit Thermo Fisher Scientific Cat# 88-7064-88 Deposited data PDAC scRNA-Seq Peng et al.35 GSA: CRA001160 RNA seq of KPC treated with TGF-β1 Hou et al.39 GSE149130 Original proteomic data Tables S5 and S6 This paper Tables S5 and S6 (Continued on next page) e3 Cell Reports Medicine 7, 102660, March 17, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines 293FT Cell Resource Center, Peking Union Medical College RRID: CVCL_6911 hTERT-HPNE ATCC RRID: CVCL_C466
Isolation:Article Title: Cancer cell-derived sialylated IgG interacting with Siglec-7/9/10 is a potential immunotherapeutic target in pancreatic cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human IFN-γ Peprotech Cat# 300-02-20 Human M-CSF Peprotech Cat# 300-25-2 Mouse M-CSF Peprotech Cat# 315-03-5 Human EGF Peprotech Cat# AF-100-15 Human FGF-basic (FGF-2/bFGF) Peprotech Cat# 100-18B B27 Gibco Cat# 17504044 Collagenase IV Solarbio Cat# C8160 DNase I Solarbio Cat# D8070 PNGase F NEB Cat# P0704 α 2-3,6,8 Neuraminidase NEB Cat# P0720 α 2-3 Neuraminidase NEB Cat# P0743 Human Siglec-9, His Tag ACRO Biosystems Cat# SI9-H52H4 CFSE Thermo Fisher Scientific Cat# C34554 pHrodo Red succinimidyl ester Thermo Fisher Scientific Cat# P36600 Hoechst Solarbio Cat# C0031 TRIzol Reagent Life Technology Cat# 15596018 1×Hieff qPCR SYBR Green Master Mix Yeasen Biotechnology Cat# 11203ES08 PEI Reagent Polysciences Cat# 23966 Fetal Bovine Serum HyClone Cat# SH30396.02 RPMI 1640 Gibco Cat# 31800-022 DMEM Gibco Cat# 12100061 Cell lysates Leagene Cat# PS0013 10mM sodium acetate pH4.5 Cytiva Cat# BR-1003-50 10mM sodium acetate pH5.5 Cytiva Cat# BR-1003-52 10mM Glycine-HCl Cytiva Cat# BR-1003-56 50mM NaOH Cytiva Cat# BR-1003-58 PBS-P+ Buffer 10× Cytiva Cat# 28995084 Critical commercial assays Opal 6-Plex Manual Detection Kit Akoya Biosciences Cat# NEL811001KT DAB Detection Kit Gene Tech Cat# GK600705 Protein G Sepharose 4 Fast Flow column GE Healthcare Cat# 17-0618-01 CNBr activated Sepharose 4B71-7086-00 AF GE Healthcare Cat# 71-7086-00 AF EasySep Human CD14 Positive Selection Kit II StemCell Technologies Cat# 17858 RevertAid First Stand cDNA Synthesis Kit Thermo Fisher Scientific Cat# K1622 Dynabeads His-Tag Isolation and Pulldown Thermo Fisher Scientific Cat# 10103D Clophosome-A and Control Liposomes FormuMax Cat# F70101C-NC-10 Amino Conjugation Kit Cytiva Cat# BR-1000-50 EZ-Link Sulfo-NHS-LC-Biotinylation Kit Thermo Fisher Scientific Cat# 21435 Lipofectamine 3000 Thermo Fisher Scientific Cat# L-3000015 LEGEND MAX Mouse IL-10 ELISA Kit Biolegend Cat# 431417 Human/Mouse TGF beta-1 Uncoated ELISA Kit Thermo Fisher Scientific Cat# 88-8350-88 Mouse IL-6 Uncoated ELISA Kit Thermo Fisher Scientific Cat# 88-7064-88 Deposited data PDAC scRNA-Seq Peng et al.35 GSA: CRA001160 RNA seq of KPC treated with TGF-β1 Hou et al.39 GSE149130 Original proteomic data Tables S5 and S6 This paper Tables S5 and S6 (Continued on next page) e3 Cell Reports Medicine 7, 102660, March 17, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines 293FT Cell Resource Center, Peking Union Medical College RRID: CVCL_6911 hTERT-HPNE ATCC RRID: CVCL_C466
Control:Article Title: Cancer cell-derived sialylated IgG interacting with Siglec-7/9/10 is a potential immunotherapeutic target in pancreatic cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human IFN-γ Peprotech Cat# 300-02-20 Human M-CSF Peprotech Cat# 300-25-2 Mouse M-CSF Peprotech Cat# 315-03-5 Human EGF Peprotech Cat# AF-100-15 Human FGF-basic (FGF-2/bFGF) Peprotech Cat# 100-18B B27 Gibco Cat# 17504044 Collagenase IV Solarbio Cat# C8160 DNase I Solarbio Cat# D8070 PNGase F NEB Cat# P0704 α 2-3,6,8 Neuraminidase NEB Cat# P0720 α 2-3 Neuraminidase NEB Cat# P0743 Human Siglec-9, His Tag ACRO Biosystems Cat# SI9-H52H4 CFSE Thermo Fisher Scientific Cat# C34554 pHrodo Red succinimidyl ester Thermo Fisher Scientific Cat# P36600 Hoechst Solarbio Cat# C0031 TRIzol Reagent Life Technology Cat# 15596018 1×Hieff qPCR SYBR Green Master Mix Yeasen Biotechnology Cat# 11203ES08 PEI Reagent Polysciences Cat# 23966 Fetal Bovine Serum HyClone Cat# SH30396.02 RPMI 1640 Gibco Cat# 31800-022 DMEM Gibco Cat# 12100061 Cell lysates Leagene Cat# PS0013 10mM sodium acetate pH4.5 Cytiva Cat# BR-1003-50 10mM sodium acetate pH5.5 Cytiva Cat# BR-1003-52 10mM Glycine-HCl Cytiva Cat# BR-1003-56 50mM NaOH Cytiva Cat# BR-1003-58 PBS-P+ Buffer 10× Cytiva Cat# 28995084 Critical commercial assays Opal 6-Plex Manual Detection Kit Akoya Biosciences Cat# NEL811001KT DAB Detection Kit Gene Tech Cat# GK600705 Protein G Sepharose 4 Fast Flow column GE Healthcare Cat# 17-0618-01 CNBr activated Sepharose 4B71-7086-00 AF GE Healthcare Cat# 71-7086-00 AF EasySep Human CD14 Positive Selection Kit II StemCell Technologies Cat# 17858 RevertAid First Stand cDNA Synthesis Kit Thermo Fisher Scientific Cat# K1622 Dynabeads His-Tag Isolation and Pulldown Thermo Fisher Scientific Cat# 10103D Clophosome-A and Control Liposomes FormuMax Cat# F70101C-NC-10 Amino Conjugation Kit Cytiva Cat# BR-1000-50 EZ-Link Sulfo-NHS-LC-Biotinylation Kit Thermo Fisher Scientific Cat# 21435 Lipofectamine 3000 Thermo Fisher Scientific Cat# L-3000015 LEGEND MAX Mouse IL-10 ELISA Kit Biolegend Cat# 431417 Human/Mouse TGF beta-1 Uncoated ELISA Kit Thermo Fisher Scientific Cat# 88-8350-88 Mouse IL-6 Uncoated ELISA Kit Thermo Fisher Scientific Cat# 88-7064-88 Deposited data PDAC scRNA-Seq Peng et al.35 GSA: CRA001160 RNA seq of KPC treated with TGF-β1 Hou et al.39 GSE149130 Original proteomic data Tables S5 and S6 This paper Tables S5 and S6 (Continued on next page) e3 Cell Reports Medicine 7, 102660, March 17, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines 293FT Cell Resource Center, Peking Union Medical College RRID: CVCL_6911 hTERT-HPNE ATCC RRID: CVCL_C466
Liposomes:Article Title: Cancer cell-derived sialylated IgG interacting with Siglec-7/9/10 is a potential immunotherapeutic target in pancreatic cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human IFN-γ Peprotech Cat# 300-02-20 Human M-CSF Peprotech Cat# 300-25-2 Mouse M-CSF Peprotech Cat# 315-03-5 Human EGF Peprotech Cat# AF-100-15 Human FGF-basic (FGF-2/bFGF) Peprotech Cat# 100-18B B27 Gibco Cat# 17504044 Collagenase IV Solarbio Cat# C8160 DNase I Solarbio Cat# D8070 PNGase F NEB Cat# P0704 α 2-3,6,8 Neuraminidase NEB Cat# P0720 α 2-3 Neuraminidase NEB Cat# P0743 Human Siglec-9, His Tag ACRO Biosystems Cat# SI9-H52H4 CFSE Thermo Fisher Scientific Cat# C34554 pHrodo Red succinimidyl ester Thermo Fisher Scientific Cat# P36600 Hoechst Solarbio Cat# C0031 TRIzol Reagent Life Technology Cat# 15596018 1×Hieff qPCR SYBR Green Master Mix Yeasen Biotechnology Cat# 11203ES08 PEI Reagent Polysciences Cat# 23966 Fetal Bovine Serum HyClone Cat# SH30396.02 RPMI 1640 Gibco Cat# 31800-022 DMEM Gibco Cat# 12100061 Cell lysates Leagene Cat# PS0013 10mM sodium acetate pH4.5 Cytiva Cat# BR-1003-50 10mM sodium acetate pH5.5 Cytiva Cat# BR-1003-52 10mM Glycine-HCl Cytiva Cat# BR-1003-56 50mM NaOH Cytiva Cat# BR-1003-58 PBS-P+ Buffer 10× Cytiva Cat# 28995084 Critical commercial assays Opal 6-Plex Manual Detection Kit Akoya Biosciences Cat# NEL811001KT DAB Detection Kit Gene Tech Cat# GK600705 Protein G Sepharose 4 Fast Flow column GE Healthcare Cat# 17-0618-01 CNBr activated Sepharose 4B71-7086-00 AF GE Healthcare Cat# 71-7086-00 AF EasySep Human CD14 Positive Selection Kit II StemCell Technologies Cat# 17858 RevertAid First Stand cDNA Synthesis Kit Thermo Fisher Scientific Cat# K1622 Dynabeads His-Tag Isolation and Pulldown Thermo Fisher Scientific Cat# 10103D Clophosome-A and Control Liposomes FormuMax Cat# F70101C-NC-10 Amino Conjugation Kit Cytiva Cat# BR-1000-50 EZ-Link Sulfo-NHS-LC-Biotinylation Kit Thermo Fisher Scientific Cat# 21435 Lipofectamine 3000 Thermo Fisher Scientific Cat# L-3000015 LEGEND MAX Mouse IL-10 ELISA Kit Biolegend Cat# 431417 Human/Mouse TGF beta-1 Uncoated ELISA Kit Thermo Fisher Scientific Cat# 88-8350-88 Mouse IL-6 Uncoated ELISA Kit Thermo Fisher Scientific Cat# 88-7064-88 Deposited data PDAC scRNA-Seq Peng et al.35 GSA: CRA001160 RNA seq of KPC treated with TGF-β1 Hou et al.39 GSE149130 Original proteomic data Tables S5 and S6 This paper Tables S5 and S6 (Continued on next page) e3 Cell Reports Medicine 7, 102660, March 17, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines 293FT Cell Resource Center, Peking Union Medical College RRID: CVCL_6911 hTERT-HPNE ATCC RRID: CVCL_C466
Conjugation Assay:Article Title: Cancer cell-derived sialylated IgG interacting with Siglec-7/9/10 is a potential immunotherapeutic target in pancreatic cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human IFN-γ Peprotech Cat# 300-02-20 Human M-CSF Peprotech Cat# 300-25-2 Mouse M-CSF Peprotech Cat# 315-03-5 Human EGF Peprotech Cat# AF-100-15 Human FGF-basic (FGF-2/bFGF) Peprotech Cat# 100-18B B27 Gibco Cat# 17504044 Collagenase IV Solarbio Cat# C8160 DNase I Solarbio Cat# D8070 PNGase F NEB Cat# P0704 α 2-3,6,8 Neuraminidase NEB Cat# P0720 α 2-3 Neuraminidase NEB Cat# P0743 Human Siglec-9, His Tag ACRO Biosystems Cat# SI9-H52H4 CFSE Thermo Fisher Scientific Cat# C34554 pHrodo Red succinimidyl ester Thermo Fisher Scientific Cat# P36600 Hoechst Solarbio Cat# C0031 TRIzol Reagent Life Technology Cat# 15596018 1×Hieff qPCR SYBR Green Master Mix Yeasen Biotechnology Cat# 11203ES08 PEI Reagent Polysciences Cat# 23966 Fetal Bovine Serum HyClone Cat# SH30396.02 RPMI 1640 Gibco Cat# 31800-022 DMEM Gibco Cat# 12100061 Cell lysates Leagene Cat# PS0013 10mM sodium acetate pH4.5 Cytiva Cat# BR-1003-50 10mM sodium acetate pH5.5 Cytiva Cat# BR-1003-52 10mM Glycine-HCl Cytiva Cat# BR-1003-56 50mM NaOH Cytiva Cat# BR-1003-58 PBS-P+ Buffer 10× Cytiva Cat# 28995084 Critical commercial assays Opal 6-Plex Manual Detection Kit Akoya Biosciences Cat# NEL811001KT DAB Detection Kit Gene Tech Cat# GK600705 Protein G Sepharose 4 Fast Flow column GE Healthcare Cat# 17-0618-01 CNBr activated Sepharose 4B71-7086-00 AF GE Healthcare Cat# 71-7086-00 AF EasySep Human CD14 Positive Selection Kit II StemCell Technologies Cat# 17858 RevertAid First Stand cDNA Synthesis Kit Thermo Fisher Scientific Cat# K1622 Dynabeads His-Tag Isolation and Pulldown Thermo Fisher Scientific Cat# 10103D Clophosome-A and Control Liposomes FormuMax Cat# F70101C-NC-10 Amino Conjugation Kit Cytiva Cat# BR-1000-50 EZ-Link Sulfo-NHS-LC-Biotinylation Kit Thermo Fisher Scientific Cat# 21435 Lipofectamine 3000 Thermo Fisher Scientific Cat# L-3000015 LEGEND MAX Mouse IL-10 ELISA Kit Biolegend Cat# 431417 Human/Mouse TGF beta-1 Uncoated ELISA Kit Thermo Fisher Scientific Cat# 88-8350-88 Mouse IL-6 Uncoated ELISA Kit Thermo Fisher Scientific Cat# 88-7064-88 Deposited data PDAC scRNA-Seq Peng et al.35 GSA: CRA001160 RNA seq of KPC treated with TGF-β1 Hou et al.39 GSE149130 Original proteomic data Tables S5 and S6 This paper Tables S5 and S6 (Continued on next page) e3 Cell Reports Medicine 7, 102660, March 17, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines 293FT Cell Resource Center, Peking Union Medical College RRID: CVCL_6911 hTERT-HPNE ATCC RRID: CVCL_C466
Enzyme-linked Immunosorbent Assay:Article Title: Cancer cell-derived sialylated IgG interacting with Siglec-7/9/10 is a potential immunotherapeutic target in pancreatic cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human IFN-γ Peprotech Cat# 300-02-20 Human M-CSF Peprotech Cat# 300-25-2 Mouse M-CSF Peprotech Cat# 315-03-5 Human EGF Peprotech Cat# AF-100-15 Human FGF-basic (FGF-2/bFGF) Peprotech Cat# 100-18B B27 Gibco Cat# 17504044 Collagenase IV Solarbio Cat# C8160 DNase I Solarbio Cat# D8070 PNGase F NEB Cat# P0704 α 2-3,6,8 Neuraminidase NEB Cat# P0720 α 2-3 Neuraminidase NEB Cat# P0743 Human Siglec-9, His Tag ACRO Biosystems Cat# SI9-H52H4 CFSE Thermo Fisher Scientific Cat# C34554 pHrodo Red succinimidyl ester Thermo Fisher Scientific Cat# P36600 Hoechst Solarbio Cat# C0031 TRIzol Reagent Life Technology Cat# 15596018 1×Hieff qPCR SYBR Green Master Mix Yeasen Biotechnology Cat# 11203ES08 PEI Reagent Polysciences Cat# 23966 Fetal Bovine Serum HyClone Cat# SH30396.02 RPMI 1640 Gibco Cat# 31800-022 DMEM Gibco Cat# 12100061 Cell lysates Leagene Cat# PS0013 10mM sodium acetate pH4.5 Cytiva Cat# BR-1003-50 10mM sodium acetate pH5.5 Cytiva Cat# BR-1003-52 10mM Glycine-HCl Cytiva Cat# BR-1003-56 50mM NaOH Cytiva Cat# BR-1003-58 PBS-P+ Buffer 10× Cytiva Cat# 28995084 Critical commercial assays Opal 6-Plex Manual Detection Kit Akoya Biosciences Cat# NEL811001KT DAB Detection Kit Gene Tech Cat# GK600705 Protein G Sepharose 4 Fast Flow column GE Healthcare Cat# 17-0618-01 CNBr activated Sepharose 4B71-7086-00 AF GE Healthcare Cat# 71-7086-00 AF EasySep Human CD14 Positive Selection Kit II StemCell Technologies Cat# 17858 RevertAid First Stand cDNA Synthesis Kit Thermo Fisher Scientific Cat# K1622 Dynabeads His-Tag Isolation and Pulldown Thermo Fisher Scientific Cat# 10103D Clophosome-A and Control Liposomes FormuMax Cat# F70101C-NC-10 Amino Conjugation Kit Cytiva Cat# BR-1000-50 EZ-Link Sulfo-NHS-LC-Biotinylation Kit Thermo Fisher Scientific Cat# 21435 Lipofectamine 3000 Thermo Fisher Scientific Cat# L-3000015 LEGEND MAX Mouse IL-10 ELISA Kit Biolegend Cat# 431417 Human/Mouse TGF beta-1 Uncoated ELISA Kit Thermo Fisher Scientific Cat# 88-8350-88 Mouse IL-6 Uncoated ELISA Kit Thermo Fisher Scientific Cat# 88-7064-88 Deposited data PDAC scRNA-Seq Peng et al.35 GSA: CRA001160 RNA seq of KPC treated with TGF-β1 Hou et al.39 GSE149130 Original proteomic data Tables S5 and S6 This paper Tables S5 and S6 (Continued on next page) e3 Cell Reports Medicine 7, 102660, March 17, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines 293FT Cell Resource Center, Peking Union Medical College RRID: CVCL_6911 hTERT-HPNE ATCC RRID: CVCL_C466
RNA Sequencing:Article Title: Cancer cell-derived sialylated IgG interacting with Siglec-7/9/10 is a potential immunotherapeutic target in pancreatic cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human IFN-γ Peprotech Cat# 300-02-20 Human M-CSF Peprotech Cat# 300-25-2 Mouse M-CSF Peprotech Cat# 315-03-5 Human EGF Peprotech Cat# AF-100-15 Human FGF-basic (FGF-2/bFGF) Peprotech Cat# 100-18B B27 Gibco Cat# 17504044 Collagenase IV Solarbio Cat# C8160 DNase I Solarbio Cat# D8070 PNGase F NEB Cat# P0704 α 2-3,6,8 Neuraminidase NEB Cat# P0720 α 2-3 Neuraminidase NEB Cat# P0743 Human Siglec-9, His Tag ACRO Biosystems Cat# SI9-H52H4 CFSE Thermo Fisher Scientific Cat# C34554 pHrodo Red succinimidyl ester Thermo Fisher Scientific Cat# P36600 Hoechst Solarbio Cat# C0031 TRIzol Reagent Life Technology Cat# 15596018 1×Hieff qPCR SYBR Green Master Mix Yeasen Biotechnology Cat# 11203ES08 PEI Reagent Polysciences Cat# 23966 Fetal Bovine Serum HyClone Cat# SH30396.02 RPMI 1640 Gibco Cat# 31800-022 DMEM Gibco Cat# 12100061 Cell lysates Leagene Cat# PS0013 10mM sodium acetate pH4.5 Cytiva Cat# BR-1003-50 10mM sodium acetate pH5.5 Cytiva Cat# BR-1003-52 10mM Glycine-HCl Cytiva Cat# BR-1003-56 50mM NaOH Cytiva Cat# BR-1003-58 PBS-P+ Buffer 10× Cytiva Cat# 28995084 Critical commercial assays Opal 6-Plex Manual Detection Kit Akoya Biosciences Cat# NEL811001KT DAB Detection Kit Gene Tech Cat# GK600705 Protein G Sepharose 4 Fast Flow column GE Healthcare Cat# 17-0618-01 CNBr activated Sepharose 4B71-7086-00 AF GE Healthcare Cat# 71-7086-00 AF EasySep Human CD14 Positive Selection Kit II StemCell Technologies Cat# 17858 RevertAid First Stand cDNA Synthesis Kit Thermo Fisher Scientific Cat# K1622 Dynabeads His-Tag Isolation and Pulldown Thermo Fisher Scientific Cat# 10103D Clophosome-A and Control Liposomes FormuMax Cat# F70101C-NC-10 Amino Conjugation Kit Cytiva Cat# BR-1000-50 EZ-Link Sulfo-NHS-LC-Biotinylation Kit Thermo Fisher Scientific Cat# 21435 Lipofectamine 3000 Thermo Fisher Scientific Cat# L-3000015 LEGEND MAX Mouse IL-10 ELISA Kit Biolegend Cat# 431417 Human/Mouse TGF beta-1 Uncoated ELISA Kit Thermo Fisher Scientific Cat# 88-8350-88 Mouse IL-6 Uncoated ELISA Kit Thermo Fisher Scientific Cat# 88-7064-88 Deposited data PDAC scRNA-Seq Peng et al.35 GSA: CRA001160 RNA seq of KPC treated with TGF-β1 Hou et al.39 GSE149130 Original proteomic data Tables S5 and S6 This paper Tables S5 and S6 (Continued on next page) e3 Cell Reports Medicine 7, 102660, March 17, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines 293FT Cell Resource Center, Peking Union Medical College RRID: CVCL_6911 hTERT-HPNE ATCC RRID: CVCL_C466
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